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. 2003 May;47(5):1766-70.
doi: 10.1128/AAC.47.5.1766-1770.2003.

Use of high-performance liquid chromatography to study the pharmacokinetics of colistin sulfate in rats following intravenous administration

Jian Li  1 , Robert W Milne, Roger L Nation, John D Turnidge, Timothy C Smeaton, Kingsley Coulthard
Affiliations

Use of high-performance liquid chromatography to study the pharmacokinetics of colistin sulfate in rats following intravenous administration

Jian Li et al. Antimicrob Agents Chemother. 2003 May.

Abstract

The pharmacokinetics of colistin was investigated using specific high-performance liquid chromatography (HPLC) to measure the concentrations of colistin and colistin A and B in plasma and urine in five rats after administration of an intravenous bolus of 1 mg of colistin sulfate/kg of body weight. There were differences in the pharmacokinetic behaviors of unbound colistin A and B. This is the first report of the use of HPLC to study the pharmacokinetics of colistin and its two major components.

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Figures

FIG. 1.
FIG. 1.
Chemical structures of colistin A and B. Fatty acid: 6-methyloctanoic acid for colistin A and 6-methylheptanoic acid for colistin B. Thr, threonine; Leu, leucine; Dab, α,γ-diaminobutyric acid. α and γ indicate the respective −NH2s involved in the peptide linkage.
FIG. 2.
FIG. 2.
Mean (±SD) plasma concentration versus time profiles for colistin and colistin A and B (all as the sulfate) in rats (n = 5).
See this image and copyright information in PMC

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