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Prof. Hesham N Mustafa

King AbdulAziz University (KAU) Jeddah, Saudi Arabia, Anatomy and Histology, Faculty Member

Neurotoxicity +60

ResearcherID: http://www.researcherid.com/rid/H-5618-2011

ORCID: http://orcid.org/0000-0003-1188-2187

https://www.researchgate.net/profile/Hesham_Mustafa
Supervisors: Member of editorial Board of the International Journal of Clinical Endocrinology and Metabolism and Member of Editorial Board of the Peertechz Journal of Cytology and Pathology
Phone: +966566764762
Address: Saudi Arabia, Egypt

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Papers

Immunohistochemical Study of the Ameliorative Effect of Vitamin E on Liver Regeneration after Different Periods of Partial Hepatectomy

Biomedical and Pharmacology Journal, 2018

The liver is almost unique in its capacity for regeneration after hepatectomy but the exact mecha... more The liver is almost unique in its capacity for regeneration after hepatectomy but the exact mechanisms are not yet fully clarified. Antioxidants have been shown to promote liver regeneration after major hepatectomy. The present study evaluated the ameliorative effect of vitamin E administration on the liver regeneration after different periods of partial hepatectomy (PH) in rats. Fifty-six adult male albino rats were divided into three groups: Control sham operated group; partially hepatectomized group which were divided into three subgroups sacrificed at 1day, 3 days and 7days after the operation respectively; Partially Hepatectomized group with vitamin E pretreatment before PH where the rats were given a daily oral dose of vitamin E until the time of sacrifice of the rats. Immunohistochemical detection of proliferating cell nuclear antigen (PCNA) and labeling index were demonstrated. After PH, the PCNA positive hepatocytes and the PCNA labeling indices were significantly high afte...

Specimen Preparation for Scanning Electron Microscopy

Tissue Culture Techniques for Horticultural Crops, 1989

Scanning electron microscopy (SEM) has become a useful tool for examining the external characteri... more Scanning electron microscopy (SEM) has become a useful tool for examining the external characteristics related to various developmental processes in plants. With SEM, the external fractures of a tissue can be observed with a greater depth of field and at a higher magnification and resolution than with light microscopy.

Preparation of Biological Specimens for Electron Microscopy

Norelco reporter, 1960

Preparation of biological specimens for Electron Microscopy

Potential Alleviation of Chlorella vulgaris and Zingiber officinale on Lead-Induced Testicular Toxicity: an Ultrastructural Study

Folia Biologica, 2015

The Ameliorative Potentials of Hyphaene thebaica on Streptozotocin Induced Diabetic Nephropathy. (Folia Morphologica)

Liver ischemia/reperfusion injury, a setting in which the functional mass is reduced and the role of PDE5 inhibitor

Liver ischemia reperfusion is induced during sur-gical procedures like liver transplantation and ... more Liver ischemia reperfusion is induced during sur-gical procedures like liver transplantation and re-section. Multiple mechanisms have been postulat-ed to liver damage following liver ischemia reperfu-sion injury, such as oxidative stress and inflamma-tory reactions. The present study declares the pos-sible mechanism of tadalafil, toward modulating the inflammatory response. Forty-eight rats were divided into 4 groups as follows; Sham group sub-jected to midline laparotomy only. Tadalafil group administered Tadalafil 10 mg/kg intraperitoneal 45 min before sham operation. I/R (Ischemia-reperfusion) group, rats undergo 60 min of hepatic ischemia followed by 60 min of reperfusion. Tada-lafil + I/R group rats undergo a similar pattern of I/R after the treatment with Tadalafil 10 mg/kg, 45 min before ischemia. At the end of the reperfusion, the blood samples were collected for estimation of biochemical markers including liver enzymes using colorimetric assay method and serum: TNF-α (tumor...

XXIV International Symposium on Morphological Sciences Abstract Book 3-9-2015

XXIV International Symposium on Morphological Sciences Abstract Book 3-9-2015

Morphohistometric analysis of the effects of Coriandrum sativum on cortical and cerebellar neurotoxicity

avicenna journal of phytomedicine, 2021

Objective: Natural compounds can act as metal chelators and oxygen free radical scavengers, which... more Objective: Natural compounds can act as metal chelators and oxygen free radical scavengers, which allows them to be used as bioactive antagonists to heavy metals neurotoxicity. The aim of the study to analyze the morphometric effects of Coriandrum sativum (C. sativum) on lead-induced neurotoxicity. Materials and Methods: Forty Sprague-Dawley albino rats were divided into four equal groups (ten in each group): control group; coriander group: received aqueous C. sativum extracts (600 mg/kg BW for 60 days orally); lead (Pb) group: received a daily dose of lead acetate (Pb) (10 mg/kg BW for 60 days orally); Pb+ coriandrum group: received: aqueous C. sativum extract (600 mg/kg BW) prior to 10 mg/kg BW of Pb. The following parameters malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) were measured. Layers thickness and nuclei density were analyzed. Results: Lead levels in blood and tissues were decreased significantly in the Pb group and tho...

Chlorella vulgaris alleviates lead-induced testicular toxicity better than Zingiber officinale : an ultrastructural study

Anterolateral ligament in pediatric knees: A radiographic study

Current Orthopaedic Practice, 2020

Background: The anterolateral ligament (ALL) is a true well-defined ligament in the knee first de... more Background: The anterolateral ligament (ALL) is a true well-defined ligament in the knee first described in 1879 by Segond. After the work of Claes et al., several studies were conducted about biomechanics and its role in stability of the knee. The anatomical existence of the ALL has been studied by and various radiographic diagnostic modalities and in cadavers. It originates from lateral femoral epicondyle and is inserted between Gerdy’s tubercle and the fibular head. There has been controversy about the existence of ALL in pediatric patients. The aim of this work was to confirm the presence of ALL in pediatric patients by using MRI. Materials and Methods: We reviewed the knee MRI scans of 100 pediatric patients (ages between one and 12 yr) who had no knee injury or congenital deformity and had been evaluated by an expert radiologist. Results: The ALL was detected in 90% of the pediatric patients with the use of MRI. Conclusions: The main finding of this study was that ALL can be seen in pediatric patients using MRI. Despite numerous studies, additional research is needed to further define the role of the ALL in knee function. Level of Evidence: Level IV.

$Research paper thumbnail of One-year mortality rate after hip fracture in the western region of Saudi Arabia$

One-year mortality rate after hip fracture in the western region of Saudi Arabia

Current Orthopaedic Practice, 2020

The Ameliorative Potential of Dexmedetomidine and Benincasa Cerifera Extract in Renal Ischemia/Reperfusion Injury in A Streptozotocin-Induced Diabetic Model

Biomedical and Pharmacology Journal, 2018

Correlation between ACL injury and involvement of the anterolateral ligament

Current Orthopaedic Practice, 2019

Biomarkers of Systemic Lupus Erythematosus and Systemic Sclerosis diseases activity in a sample of Egyptian patients :Soluble Intercellular Adhesion Molecule-1 and Soluble Interleukin-2 Receptor, Case Control Study

Biomedical & Pharmacology Journal, 2019

Systemic Lupus Erythematosus (SLE) and systemic sclerosis (SSc) are systemic inflammatory autoimm... more Systemic Lupus Erythematosus (SLE) and systemic sclerosis (SSc) are systemic inflammatory autoimmune disorders characterized by a large spectrum of clinical and laboratory features. The aim of the present study was to investigate the possible use of serum level of soluble intercellular adhesion molecule-1(sICAM-1) and soluble interleukin-2 receptor (sIL-2Ra) as biomarkers for monitoring of SLE and SSc disease activity. Moreover, it aimed to compare the specificity and sensitivity as well as cut-off value of both biomarkers in a sample of Egyptian patients. 50 SLE patients, 30 SSc patients and 60 age and sex matched healthy controls were enrolled in our study. sICAM-1and sIL-2Ra were measured in serum samples obtained from all participants. In addition to Erythosedimentation rate (ESR), complete blood count (CBC), Antineuclearantibodies (ANA) estimation, disease activity of both diseases were also assessed. sICAM-1and sIL-2Ra levels were higher in SLE and SSc patients versus control....

Thymoquinone ameliorates oxidative damage and histopathological changes of developing brain neurotoxicity

Journal of Histotechnology, 2019

Protective effect of garlic extract against maternal and fetal cerebellar damage induced by lead administration during pregnancy in rats

Folia Morphologica, 2015

Evaluation of the safety of conventional lighting replacement by artificial daylight

Journal of Microscopy and Ultrastructure, 2017

Dear Plagiarist: A Letter to a Peer Reviewer Who Stole and Published Our Manuscript as His Own

Annals of Internal Medicine, 2016

Protective role of CoQ10 or L-carnitine on the integrity of the myocardium in doxorubicin induced toxicity

Tissue and Cell, 2017

The role of curcumin in streptozotocin-induced hepatic damage and the trans-differentiation of hepatic stellate cells

Tissue & cell, 2016

Diabetic patients frequently suffer from non-alcoholic steatohepatitis. The current study aimed t... more Diabetic patients frequently suffer from non-alcoholic steatohepatitis. The current study aimed to investigate the role of curcumin and the response of hepatic stellate cells in streptozotocin (STZ)-induced hepatic damage. Sixty male rats were divided into three groups. The normal control injected with a citrate buffer vehicle and the diabetic control group which was injected intraperitoneally (IP) with a single-dose of streptozotocin (50mg/kg body weight) and a diabetic group was treated with an oral dose of curcumin at 80 mg/kg body weight daily for 60 days. Curcumin effectively counteracts oxidative stress-mediated hepatic damage and improves biochemical parameters. Alpha-smooth muscle actin (α-SMA) was significantly reduced, and insulin antibodies showed strong positive immunoreactivity with curcumin administration. These results optimistically demonstrate the potential use of curcumin, which is attributed to its antiradical/antioxidant activities and its potential β-cell regene...

Immunohistochemical Study of the Ameliorative Effect of Vitamin E on Liver Regeneration after Different Periods of Partial Hepatectomy

Biomedical and Pharmacology Journal, 2018

The liver is almost unique in its capacity for regeneration after hepatectomy but the exact mecha... more The liver is almost unique in its capacity for regeneration after hepatectomy but the exact mechanisms are not yet fully clarified. Antioxidants have been shown to promote liver regeneration after major hepatectomy. The present study evaluated the ameliorative effect of vitamin E administration on the liver regeneration after different periods of partial hepatectomy (PH) in rats. Fifty-six adult male albino rats were divided into three groups: Control sham operated group; partially hepatectomized group which were divided into three subgroups sacrificed at 1day, 3 days and 7days after the operation respectively; Partially Hepatectomized group with vitamin E pretreatment before PH where the rats were given a daily oral dose of vitamin E until the time of sacrifice of the rats. Immunohistochemical detection of proliferating cell nuclear antigen (PCNA) and labeling index were demonstrated. After PH, the PCNA positive hepatocytes and the PCNA labeling indices were significantly high afte...

Specimen Preparation for Scanning Electron Microscopy

Tissue Culture Techniques for Horticultural Crops, 1989

Scanning electron microscopy (SEM) has become a useful tool for examining the external characteri... more Scanning electron microscopy (SEM) has become a useful tool for examining the external characteristics related to various developmental processes in plants. With SEM, the external fractures of a tissue can be observed with a greater depth of field and at a higher magnification and resolution than with light microscopy.

Preparation of Biological Specimens for Electron Microscopy

Norelco reporter, 1960

Preparation of biological specimens for Electron Microscopy

Potential Alleviation of Chlorella vulgaris and Zingiber officinale on Lead-Induced Testicular Toxicity: an Ultrastructural Study

Folia Biologica, 2015

The Ameliorative Potentials of Hyphaene thebaica on Streptozotocin Induced Diabetic Nephropathy. (Folia Morphologica)

Liver ischemia/reperfusion injury, a setting in which the functional mass is reduced and the role of PDE5 inhibitor

Liver ischemia reperfusion is induced during sur-gical procedures like liver transplantation and ... more Liver ischemia reperfusion is induced during sur-gical procedures like liver transplantation and re-section. Multiple mechanisms have been postulat-ed to liver damage following liver ischemia reperfu-sion injury, such as oxidative stress and inflamma-tory reactions. The present study declares the pos-sible mechanism of tadalafil, toward modulating the inflammatory response. Forty-eight rats were divided into 4 groups as follows; Sham group sub-jected to midline laparotomy only. Tadalafil group administered Tadalafil 10 mg/kg intraperitoneal 45 min before sham operation. I/R (Ischemia-reperfusion) group, rats undergo 60 min of hepatic ischemia followed by 60 min of reperfusion. Tada-lafil + I/R group rats undergo a similar pattern of I/R after the treatment with Tadalafil 10 mg/kg, 45 min before ischemia. At the end of the reperfusion, the blood samples were collected for estimation of biochemical markers including liver enzymes using colorimetric assay method and serum: TNF-α (tumor...

XXIV International Symposium on Morphological Sciences Abstract Book 3-9-2015

XXIV International Symposium on Morphological Sciences Abstract Book 3-9-2015

Morphohistometric analysis of the effects of Coriandrum sativum on cortical and cerebellar neurotoxicity

avicenna journal of phytomedicine, 2021

Objective: Natural compounds can act as metal chelators and oxygen free radical scavengers, which... more Objective: Natural compounds can act as metal chelators and oxygen free radical scavengers, which allows them to be used as bioactive antagonists to heavy metals neurotoxicity. The aim of the study to analyze the morphometric effects of Coriandrum sativum (C. sativum) on lead-induced neurotoxicity. Materials and Methods: Forty Sprague-Dawley albino rats were divided into four equal groups (ten in each group): control group; coriander group: received aqueous C. sativum extracts (600 mg/kg BW for 60 days orally); lead (Pb) group: received a daily dose of lead acetate (Pb) (10 mg/kg BW for 60 days orally); Pb+ coriandrum group: received: aqueous C. sativum extract (600 mg/kg BW) prior to 10 mg/kg BW of Pb. The following parameters malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) were measured. Layers thickness and nuclei density were analyzed. Results: Lead levels in blood and tissues were decreased significantly in the Pb group and tho...

Chlorella vulgaris alleviates lead-induced testicular toxicity better than Zingiber officinale : an ultrastructural study

Anterolateral ligament in pediatric knees: A radiographic study

Current Orthopaedic Practice, 2020

Background: The anterolateral ligament (ALL) is a true well-defined ligament in the knee first de... more Background: The anterolateral ligament (ALL) is a true well-defined ligament in the knee first described in 1879 by Segond. After the work of Claes et al., several studies were conducted about biomechanics and its role in stability of the knee. The anatomical existence of the ALL has been studied by and various radiographic diagnostic modalities and in cadavers. It originates from lateral femoral epicondyle and is inserted between Gerdy’s tubercle and the fibular head. There has been controversy about the existence of ALL in pediatric patients. The aim of this work was to confirm the presence of ALL in pediatric patients by using MRI. Materials and Methods: We reviewed the knee MRI scans of 100 pediatric patients (ages between one and 12 yr) who had no knee injury or congenital deformity and had been evaluated by an expert radiologist. Results: The ALL was detected in 90% of the pediatric patients with the use of MRI. Conclusions: The main finding of this study was that ALL can be seen in pediatric patients using MRI. Despite numerous studies, additional research is needed to further define the role of the ALL in knee function. Level of Evidence: Level IV.

$Research paper thumbnail of One-year mortality rate after hip fracture in the western region of Saudi Arabia$

One-year mortality rate after hip fracture in the western region of Saudi Arabia

Current Orthopaedic Practice, 2020

The Ameliorative Potential of Dexmedetomidine and Benincasa Cerifera Extract in Renal Ischemia/Reperfusion Injury in A Streptozotocin-Induced Diabetic Model

Biomedical and Pharmacology Journal, 2018

Correlation between ACL injury and involvement of the anterolateral ligament

Current Orthopaedic Practice, 2019

Biomarkers of Systemic Lupus Erythematosus and Systemic Sclerosis diseases activity in a sample of Egyptian patients :Soluble Intercellular Adhesion Molecule-1 and Soluble Interleukin-2 Receptor, Case Control Study

Biomedical & Pharmacology Journal, 2019

Systemic Lupus Erythematosus (SLE) and systemic sclerosis (SSc) are systemic inflammatory autoimm... more Systemic Lupus Erythematosus (SLE) and systemic sclerosis (SSc) are systemic inflammatory autoimmune disorders characterized by a large spectrum of clinical and laboratory features. The aim of the present study was to investigate the possible use of serum level of soluble intercellular adhesion molecule-1(sICAM-1) and soluble interleukin-2 receptor (sIL-2Ra) as biomarkers for monitoring of SLE and SSc disease activity. Moreover, it aimed to compare the specificity and sensitivity as well as cut-off value of both biomarkers in a sample of Egyptian patients. 50 SLE patients, 30 SSc patients and 60 age and sex matched healthy controls were enrolled in our study. sICAM-1and sIL-2Ra were measured in serum samples obtained from all participants. In addition to Erythosedimentation rate (ESR), complete blood count (CBC), Antineuclearantibodies (ANA) estimation, disease activity of both diseases were also assessed. sICAM-1and sIL-2Ra levels were higher in SLE and SSc patients versus control....

Thymoquinone ameliorates oxidative damage and histopathological changes of developing brain neurotoxicity

Journal of Histotechnology, 2019

Protective effect of garlic extract against maternal and fetal cerebellar damage induced by lead administration during pregnancy in rats

Folia Morphologica, 2015

Evaluation of the safety of conventional lighting replacement by artificial daylight

Journal of Microscopy and Ultrastructure, 2017

Dear Plagiarist: A Letter to a Peer Reviewer Who Stole and Published Our Manuscript as His Own

Annals of Internal Medicine, 2016

Protective role of CoQ10 or L-carnitine on the integrity of the myocardium in doxorubicin induced toxicity

Tissue and Cell, 2017

The role of curcumin in streptozotocin-induced hepatic damage and the trans-differentiation of hepatic stellate cells

Tissue & cell, 2016

Diabetic patients frequently suffer from non-alcoholic steatohepatitis. The current study aimed t... more Diabetic patients frequently suffer from non-alcoholic steatohepatitis. The current study aimed to investigate the role of curcumin and the response of hepatic stellate cells in streptozotocin (STZ)-induced hepatic damage. Sixty male rats were divided into three groups. The normal control injected with a citrate buffer vehicle and the diabetic control group which was injected intraperitoneally (IP) with a single-dose of streptozotocin (50mg/kg body weight) and a diabetic group was treated with an oral dose of curcumin at 80 mg/kg body weight daily for 60 days. Curcumin effectively counteracts oxidative stress-mediated hepatic damage and improves biochemical parameters. Alpha-smooth muscle actin (α-SMA) was significantly reduced, and insulin antibodies showed strong positive immunoreactivity with curcumin administration. These results optimistically demonstrate the potential use of curcumin, which is attributed to its antiradical/antioxidant activities and its potential β-cell regene...

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Neuroprotective potential of Aqueous Cinnamon Extract on cerebellar Amyloid-β plaques in Alzheimer's Disease [P2-E/4]

Focus on Microscopy 2021, 2021

Background Aluminum (AL) is a neurotoxic substance that plays an important role in the etiology, ... more Background Aluminum (AL) is a neurotoxic substance that plays an important role in the etiology, pathogenesis and development of Amyloid-β plaques. This study was carried out to evaluate neuroprotective effect of Aqueous Cinnamon Extract against aluminum chloride (ALCl3)-induced Alzheimer's disease. Materials and methods 40 adult male albino rats, randomly divided into four equal groups. Group I: control; Group 2 were administered 200 mg/kg b. w./day aqueous cinnamon extract (ACE) via oral tube; Group 3: received daily intraperitoneal (i.p.) injection of ALCl3 (100 mg/kg body weight) for 60 days to induce neurotoxicity; Group 4 received a combination of ALCl3 and ACE in the same dose and route as group 2 & 3. Results AL administration significantly enhanced the memory impairment, Aβ burden and the cerebellum exhibited significant reduction in the number of Purkinje cells, marked decrease in the density of dendritic arborization and prominent perineuronal spaces in the molecular layer around basket and stellate cells, loss of dendritic spines, neurofibrillary degeneration and appearance of neuritic plaques. Concomitant administration of ACE with AL displayed an observable protection against these changes with progressive improvement in memory and in intellectual performance. It has also been suggested that the loss of astrocyte functions may precede neurodegeneration and that aluminum could be a contributing factor [1]. Astrocytes are the principal target of the toxic action of aluminum [2] and it can cause astrocyte death through apoptosis [3]. Conclusion: ACE may be considered an efficacious therapeutic strategy to alleviate Amyloid-β plaques. It is recommended that AL not be used in cooking utensils, water tubes, and to control occupational exposure.

CERTIFICATE OF PRESENTATION Protective Role of CoQ10 or L-Carnitine on the Integrity of the Myocardium in Doxorubicin Induced Toxicity

Doxorubicin (DOX) is a chemotherapeutic agent used for treatment of different cancers and its cli... more Doxorubicin (DOX) is a chemotherapeutic agent used for treatment of different cancers and its clinical usage is hindered by the oxidative injury-related cardiotoxicity. This work aims to declare if the harmful effects of DOX on heart can be alleviated with the use of Coenzyme Q10 (CoQ10) or L-carnitine. The study was performed on seventy two female Wistar albino rats divided into six groups, 12 animals each: Control group; DOX group (10mg/kg); CoQ10 group (200mg/kg); L-carnitine group (100mg/kg); DOX+CoQ10 group; DOX+L-carnitine group. CoQ10 and L-carnitine treatment orally started 5days before a single dose of 10mg/kg DOX that injected intraperitoneally (IP) then the treatment continued for 10days. At the end of the study, serum biochemical parameters of cardiac damage, oxidative stress indices, and histopathological changes were investigated. CoQ10 or L-carnitine showed a noticeable effects in improving cardiac functions evidenced reducing serum enzymes as serum interleukin-1 beta (IL-1 β), tumor necrosis factor alpha (TNF-α), leptin, lactate dehydrogenase (LDH), Cardiotrophin-1, Troponin-I and Troponin-T. Also, alleviate oxidative stress, decrease of cardiac Malondialdehyde (MDA), Nitric oxide (NO) and restoring cardiac reduced glutathione levels to normal levels. Both corrected the cardiac alterations histologically and ultrastructurally. With a visible improvements in α-SMA, vimentin and eNOS immunohistochemical markers. CoQ10 or L-carnitine supplementation improves the functional and structural integrity of the myocardium.

KEYWORDS:
Cardiotoxicity; CoQ10 and L-carnitine; Dox; Vimentin; eNOS

CERTIFICATE OF PRESENTATION Protective Role of CoQ10 or L-Carnitine on the Integrity of the Myocardium in Doxorubicin Induced Toxicity

20th International Conference on Biochemistry, Biophysics and Molecular Biology. Doxorubicin (DO... more 20th International Conference on Biochemistry, Biophysics and Molecular Biology.

Doxorubicin (DOX) is a chemotherapeutic agent used for treatment of different cancers and its clinical usage is hindered by the oxidative injury-related cardiotoxicity. This work aims to declare if the harmful effects of DOX on heart can be alleviated with the use of Coenzyme Q10 (CoQ10) or L-carnitine. The study was performed on seventy two female Wistar albino rats divided into six groups, 12 animals each: Control group; DOX group (10mg/kg); CoQ10 group (200mg/kg); L-carnitine group (100mg/kg); DOX+CoQ10 group; DOX+L-carnitine group. CoQ10 and L-carnitine treatment orally started 5days before a single dose of 10mg/kg DOX that injected intraperitoneally (IP) then the treatment continued for 10days. At the end of the study, serum biochemical parameters of cardiac damage, oxidative stress indices, and histopathological changes were investigated. CoQ10 or L-carnitine showed a noticeable effects in improving cardiac functions evidenced reducing serum enzymes as serum interleukin-1 beta (IL-1 β), tumor necrosis factor alpha (TNF-α), leptin, lactate dehydrogenase (LDH), Cardiotrophin-1, Troponin-I and Troponin-T. Also, alleviate oxidative stress, decrease of cardiac Malondialdehyde (MDA), Nitric oxide (NO) and restoring cardiac reduced glutathione levels to normal levels. Both corrected the cardiac alterations histologically and ultrastructurally. With a visible improvements in α-SMA, vimentin and eNOS immunohistochemical markers. CoQ10 or L-carnitine supplementation improves the functional and structural integrity of the myocardium.

KEYWORDS:
Cardiotoxicity; CoQ10 and L-carnitine; Dox; Vimentin; eNOS

15th International Congress of Histochemistry and Cytochemistry “From Molecules to Diseases”

15th International Congress of Histochemistry and Cytochemistry " From Molecules to Diseases " Ameliorative potentials of a combination of fenugreek and alpha-tocopherol on cadmium induced testicular toxicity: an ultrastructural study

BACKGROUND: The current study aimed to elucidate the protective role of combined fenugreek and a-... more BACKGROUND: The current study aimed to elucidate the protective role of combined fenugreek and a-tocopherol against cadmium induced histopathological changes in the testes. MATERIALS-METHODS: Thirty adult male albino rats divided into three equal groups 10 rats each. Group I is the control group. Group II received 5 mg/kg/ day cadmium chloride. Group III received 5 mg/kg/day cadmium chloride and 150 mg/kg/day fenugreek and 100 mg/kg/ day of a-tocopherol. The treatment of all groups was done by oral gavage for 60 consecutive days. The testes were removed and subjected to histopathological and ultrastructure study. RESULTS: Rats exposed to cadmium showed severe histopathological changes in the testicular spermatogenic series, many vacuoles and multinucleated giant cells. Treatment with fenugreek and a-tocopherol partially improved the morphological changes, reduced tissue damage and rebuilt of the spermatogonia layer. CONCLUSIONS: Fenugreek and a-tocopherol might represent a promising medicinal combination to ameliorate the toxic effects of cadmium exposure.

15th International Congress of Histochemistry and Cytochemistry " From Molecules to Diseases " The role of curcumin in streptozotocin-induced hepatic damage and the trans- differentiation of hepatic stellate cells

Diabetic patients frequently suffer from non-alcoholic steatohepatitis. The current study aimed t... more Diabetic patients frequently suffer from non-alcoholic steatohepatitis. The current study aimed to investigate the role of curcumin and the response of hepatic stellate cells in streptozotocin (STZ)-induced hepatic damage. Sixty male rats were divided into three groups. The normal control injected with a citrate buffer vehicle and the diabetic control group which was injected intraperitoneally (IP) with a single-dose of streptozotocin (50 mg/kg body weight) and a diabetic group was treated with an oral dose of curcumin at 80 mg/kg body weight daily for 60 days. Results. Curcumin effectively counteracts oxidative stress-mediated hepatic damage and improves biochemical parameters. Alpha-smooth muscle actin (α-SMA) was significantly reduced, and insulin antibodies showed strong positive immunoreactivity with curcumin administration. These results optimistically demonstrate the potential use of curcumin, which is attributed to its antiradical/ antioxidant activities and its potential β-cell regenerative properties. Also, it has the capability to encourage the trans-differentiation of hepatic stellate cells into insulin-producing cells for a period of time. In addition, as it is an anti-fibrotic mediator that inhibits hepatic stellate cell activation and the transition to myofibroblast-like cells, this suggests the possibility of considering curcumin's novel therapeutic effects in reducing hepatic dysfunction in diabetic patients. Body and liver weight of different groups. The mean is given in columns, and error bars represent the standard deviation (SD). Fig. 2 Plasma glucose and insulin of different groups. The mean is given in columns, and error bars represent the standard deviation (SD). Fig. 3 Biochemical parameters of different groups. The mean is given in columns, and error bars represent the standard deviation (SD).

Folia Morphologica Reviewers 2017

Protective role of CoQ10 or L-carnitine on the integrity of the myocardium in doxorubicin induced toxicity in Quantitative Bioimaging 2018 January 4-6, 2018, Georg-August-Universität, Göttingen, Germany

Doxorubicin (DOX) is a chemotherapeutic agent used for treatment of different cancers and its cli... more Doxorubicin (DOX) is a chemotherapeutic agent used for treatment of different cancers and its clinical usage is hindered by the oxidative injury-related cardiotoxicity. This work aims to declare if the harmful effects of DOX on heart can be alleviated with the use of Coenzyme Q10 (CoQ10) or L-carnitine. The study was performed on seventy two female Wistar albino rats divided into six groups, 12 animals each: Control group; DOX group (10 mg/kg); CoQ10 group (200 mg/kg); L-carnitine group (100 mg/kg); DOX + CoQ10 group; DOX + L-carnitine group. CoQ10 and L-carnitine treatment orally started 5 days before a single dose of 10 mg/kg DOX that injected intraperitoneally (IP) then the treatment continued for 10 days. At the end of the study, serum biochemical parameters of cardiac damage, oxidative stress indices, and histopathological changes were investigated. CoQ10 or L-carnitine showed a noticeable effects in improving cardiac functions evidenced reducing serum enzymes as serum interleukin-1 beta (IL-1β), tumor necrosis factor alpha (TNF-α), leptin, lactate dehydrogenase (LDH), Cardiotrophin-1, Troponin-I and Troponin-T. Also, alleviate oxidative stress, decrease of cardiac Malondialdehyde (MDA), Nitric oxide (NO) and restoring cardiac reduced glutathione levels to normal levels. Both corrected the cardiac alterations histologically and ultrastructurally. With a visible improvements in α-SMA, vimentin and eNOS immunohistochemical markers. In conclusion, CoQ10 or L-carnitine supplementation improves the functional and structural integrity of the myocardium.

Does allicin combined with vitamin B-complex have superior potentials than alpha-tocopherol alone in ameliorating lead acetate- induced Purkinje cell alterations in rats? An immunohistochemical and ultrastructural study

BACKGROUND: The current article aims to explore the protective potentials of α-tocopherol alone ... more BACKGROUND:

The current article aims to explore the protective potentials of α-tocopherol alone and the combination of allicin and vitamin B-complex against lead-acetate neurotoxicity on the cerebellar cortex.
MATERIALS AND METHODS:

Forty rats were divided into four groups (n=10). Group 1 was the control group. Group 2 received 10 mg/kg body weight (BW) of lead acetate. Group 3 was exposed to 10 mg/kg BW of lead acetate plus a combination of allicin (100 mg/kg BW) and vit. B-complex (40 mg/kg BW). Group 4 was administered lead acetate (10 mg/kg BW) and α-tocopherol (100 mg/kg BW). The animals received treatment for sixty days by oral gavage. All the groups were studied ultrastructurally and immunohistochemically with glial fibrillary acidic protein (GFAP).
RESULTS:

The affected groups revealed shrunken and degenerated Purkinje cells with irregular nuclei. The cytoplasm comprised several lysosomes, unhealthy mitochondria, and dilated Golgi saccules. The myelinated nerve fibers demonstrated breaking of the myelin sheaths, apparent vacuoles, and broad axonal spaces. Immunohistochemically, there was a tremendous surge in GFAP-positive astrocytes in the lead acetate-treated group. These histological and ultrastructural variations were ameliorated by the administration of α-tocopherol and the combination of allicin and vit. B complex. Moreover, an apparent decrease in the number of GFAP-positive astrocytes was obvious in the protected groups.
CONCLUSIONS:

Although both α-tocopherol and the combination of allicin and vit. B-complex can be used as possible adjuvant therapies to ameliorate nervous system ailments attributable to lead acetate, α-tocopherol showed more protective potential.
KEYWORDS:

Allicin; Astrocytes; GFAP; Myelin Figure; Oligodendrocyte; Purkinje cells

Antibodies for neuroscience research

Antibodies for neuroscience research

Antibody-based tools for neurodegenerative disease research Handbook

Antibody-based tools for neurodegenerative disease research Handbook

Tissue And Cell "Protective role of CoQ10 or L-carnitine on the integrity of the myocardium in Doxorubicin induced toxicity. "

by Prof. Hesham N Mustafa and Sally El Awdan

Tissue And Cell "Protective role of CoQ10 or L-carnitine on the integrity of the myocardium in Do... more