Phenotypic and genotypic detection of Enterobacter spp isolated from food
Keywords:
Enterobacter Cloacae Complex, RpoS, fimH, fimA, BiofilmAbstract
The alternative sigma factor is essential for bacterial survival in harsh environments. Many Enterobacter spp. are opportunistic human pathogens, and their ability to adapt to changing environments may be critical to their virulence. These specialized sigma factors bind the promoters of genes appropriate to the environmental conditions and selectively increase the transcription of those genes. We used PCR to detected presence of RpoS, fimH and fimA genes in Enterobacter Cloacae Complex that isolated from food, (50%) of the isolates contain RpoS gene, (30%) of the isolates contain fimH and (20%) of the isolates contain fimA. RpoS was an important factor in cell control and bacterial persistence under stress conditions, and it contributed to virulence through a variety of mechanisms. Biofilm-producing bacteria were identified by the Congo Red Agar (CRA) method we obtained (50%) isolated of Enterobacter Cloacae Complex (ECC) formed Biofilm, according to laboratory investigations, 10 food samples in the current study contained Enterobacter spp. and were tested for antibiotic resistance. In our study, Amoixcillin-clavulanate and Ampicillin resistance was observed in all sample, while Imipenem and Levofloxacin showed high sensitivity.
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