Abstract
MARINE bacteria often dominate the plankton biomass1,2 and are responsible for much of the cycling of organic matter3, but bacterial diversity is poorly understood because conventional identification methods (requiring culturing) miss about 99% of the organisms4,5. Recent advances permit characterization of microbial communities by analysis of 16S ribosomal RNA gene sequences directly from biomass without the need to culture the organisms6; such studies from surface ocean samples have found only eubacteria7–10, not archaebacteria (or Archae11), which are profoundly different12. Here we report 16S rRNA sequences obtained from Pacific Ocean bacterioplankton samples collected from depths of 100 m and 500 m. Among these we found sequences only distantly related to those of any organisms previously characterized by 16S rRNA sequences, with similarities to the nearest such relatives (extreme thermophiles) approximately the same as those between animals and plants. We suggest that these sequences are from a previously undescribed archaebacterial group that may have diverged from the ancestors of characterized organisms very early in evolution.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 52 print issues and online access
$199.00 per year
only $3.83 per issue
Buy this article
- Purchase on SpringerLink
- Instant access to the full article PDF.
USD 39.95
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Cho, B. C. & Azam, F. Nature 332, 441–443 (1988).
Fuhrman, J. A., Sleeter, T. D., Carlson, C. A. & Proctor, L. M. Mar. Ecol. Prog. Ser. 57, 207–217 (1989).
Azam, F. et al. Mar. Ecol. Prog. Ser. 10, 257–263 (1983).
Jannasch, H. W. & Jones, G. E. Limnol. Oceanogr. 4, 128–139 (1959).
Ferguson, R. L., Buckley, E. N. & Palumbo, A. V. Appl. envir. Microbiol. 47, 49–55 (1984).
Pace, N. R., Stahl, D. A., Lane, D. L. & Olsen, G. J. Adv. microb. Ecol. 9, 1–55 (1986).
Giovannoni, S. J., Britschgi, T. B., Moyer, C. L. & Field, K. G. Nature 345, 60–63 (1990).
Giovannoni, S. J., DeLong, E. F., Schmidt, T. M. & Pace, N. R. Appl. envir. Microbiol. 56, 2572–2575 (1990).
Britschgi, T. B. & Giovannoni, S. J. Appl. envir. Microbiol. 57, 1707–1713 (1991).
Schmidt, T. M., DeLong, E. F. & Pace, N. R. J. Bact. 173, 4371–4378 (1991).
Woese, C. R., Kandler, O. & Wheelis, M. L. Proc. natn. Acad. Sci. U.S.A. 87, 4576–4579 (1990).
Woese, C. R. Microb. Rev. 51, 221–271 (1987).
Lee, S. & Fuhrman, J. A. Limnol. Oceanogr. 36, 1277–1287 (1991).
Felsenstein, J. Evolution 39, 783–791 (1985).
Distel, D. L. et al. J. Bact. 170, 2506 (1988).
Ward, D. M., Weller, R. & Bateson, M. M. Nature 345, 63–65 (1990).
Amann, R. et al. Nature 351, 161–163 (1991).
Hobbie, J. E., Daley, R. J. & Jasper, S. Appl. environ. Microbiol. 33, 1225–1228 (1977).
Holm-Hansen, O. et al. J. Cons. perm. int. Explor. Mer. 30, 3 (1965).
Lee, S. & Fuhrman, J. A. Appl. envir. Microbiol. 56, 739–746 (1990).
Fuhrman, J. A., Comeau, D. E., Hagstrom,. & Chan, A. M. Appl. envir. Microbiol. 54, 1426–1429 (1988).
Saiki, R. K. et al. Science 230, 1350–1354 (1985).
Lane, D. J. et al. Proc. natn. Acad. Sci. U.S.A. 82, 6955–6959 (1985).
Maniatis, T., Fritsch, E. F. & Sambrook, J. Molecular Cloning: a Laboratory Manual (Cold Spring Harbor, New York, 1982).
Olsen, G. J. Meth. Enzym. 164, 793–812 (1988).
Bilofsky, H. S. & Burks, C. Nucleic Acids Res. 16, 1861 (1988).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Fuhrman, J., McCallum, K. & Davis, A. Novel major archaebacterial group from marine plankton. Nature 356, 148–149 (1992). https://doi.org/10.1038/356148a0
Received:
Accepted:
Issue date:
DOI: https://doi.org/10.1038/356148a0
