Abstract
Cross polarization/magic angle spinning (CP/MAS)13C (solid state high resolution) NMR spectra were observed for chlorosomes and BChlc aggregates. Similarity of both kinds of spectra (except for some signals assignable to proteins and lipids in chlorosomes) indicates that BChlc's in chlorosomes are present just as in synthetic BChlc aggregates. Chemical shifts for C131 carbonyl and C31 hydroxylethyl carbons indicate hydrogen bonding between them. Comparison of solution and solid state13C NMR chemical shifts shows the five coordinated nature of BChlc aggregates. Some chemical shift differences were attributable to ring currents shifts. Their comparisons with calculated ring current shift values predicted structures for the aggregates. Cross polarization dynamics of the CP/MAS13C NMR signals explored dynamic and structural nature of the BChlc aggregates.
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Abbreviations
- BChl:
-
bacteriochlorophyll
- CP:
-
cross polarization
- MAS:
-
magic angle spinning
- TMS:
-
tetramethylsilane
- TOSS:
-
total elimination of spinning side bands
- TCH :
-
cross polarization time constant between C and H
- T1ϱH:
-
spin lattice relaxation time in the rotating frame
- τ:
-
contact time
- τm :
-
mixing time
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Nozawa, T., Ohtomo, K., Suzuki, M. et al. Structures of chlorosomes and aggregated BChlc inChlorobium tepidum from solid state high resolution CP/MAS13C NMR. Photosynth Res 41, 211–223 (1994). https://doi.org/10.1007/BF02184162
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DOI: https://doi.org/10.1007/BF02184162
